Glycolysis and Pyruvate Oxidation
Glycolysis is your body's first step in extracting energy from glucose, and it happens right in the cytoplasm of your cells. This 10-step biochemical pathway converts one glucose molecule (6 carbon atoms) into two pyruvate molecules (3 carbon atoms each).
The process occurs in three main stages: energy investment, cleavage reactions, and energy production. During the energy investment phase, the cell actually uses 2 ATP molecules to "prime" the glucose. This investment pays off later when the pathway generates 4 ATP molecules, giving a net gain of 2 ATP. Glycolysis also produces 2 NADH molecules, which store additional energy.
What happens to pyruvate next depends entirely on oxygen availability. When oxygen is present, pyruvate moves into the mitochondria and undergoes pyruvate oxidation. During this process, each pyruvate molecule loses a carbon atom (released as CO₂), and the remaining two-carbon fragment attaches to Coenzyme A to form acetyl-CoA. This reaction also produces another NADH molecule.
Connection Alert: Think of pyruvate as being at a metabolic crossroads. With oxygen, it enters the high-energy pathway of the Krebs cycle. Without oxygen, your cells resort to fermentation, which produces much less ATP but allows glycolysis to continue.
The acetyl-CoA now enters the Krebs cycle (also called the citric acid cycle), which occurs in the mitochondrial matrix. This cycle begins when the acetyl group combines with oxaloacetate to form citrate, and then proceeds through several steps. For each glucose molecule, the Krebs cycle produces 2 ATP, reduces 6 NAD+ to 6 NADH, and reduces 2 FAD to 2 FADH₂. It also releases the remaining carbon atoms as CO₂.